The Penn Vet Imaging Core (PVIC) provides access to cutting-edge optical imaging capabilities for researchers at the University of Pennsylvania, Children's Hospital of Philadelphia, and Wistar Institute.
The PVIC includes instruments to perform widefield, confocal, multiphoton, fluorescence lifetime, and total internal reflection fluorescence (TIRF) microscopy, as well as software tools for image analysis.
Ruthel, Gordon., PhD
Role: Core Manger, Penn Vet Imaging Facility
A single copy of Huygens Essential deconvolution/ image restoration software by Scientific Volume Imaging is available for use with Leica LIF files on the workstation computer in the core manager’s office. This software can be used on image stacks taken on the core’s Leica DM6000B widefield epifluorescence microscope to remove out of focus haze from the images to yield almost confocal quality images. Image stacks taken with the core’s Leica SP5 or SP8 confocal microscopes can be deconvolved to yield super-resolution images, provided image acquisition was optimized to make the most of the available optical resolution.
The core has a single license for Bitplane’s Imaris image analysis software, which is available on the workstation computer in the core manager’s office room 313 Hill Pavilion. The license includes the following modules: Imaris, ImarisMeasurementPro, ImarisTrack, ImarisColoc, ImarisVantage, ImarisCell, and ImarisXT. An alternative to Volocity, this is a visualization and analysis software for 3-dimensional and 4-dimensional datasets that provides an interface with MatLab via the ImarisXT module.
The core has two copies of MetaMorph: one on the computer associated with the Yokagawa Spinning Disk Confocal and one in the core manager’s office room 313 Hill Pavilion. Although there is substantial overlap in the features within these tow copies, the version in 313 Hill contains some additional analysis features not present on the version used for acquisition on the Spinning Disk Confocal.
SimFCS is software developed by Enrico Gratton of the Laboratory for Fluorescence Dynamics (LFD) and can be used to produce Phasor plots of FLIM data and analyze FRET interactions, analyze Fluorescence Correlation Spectroscopy (FCS) data, and for single particle tracking. SimFCS is available on the computer associated with the FLIM microscope and in the core manager’s office (Hill 313). Tutorials on SimFCS are available on the LFD website:http://www.lfd.uci.edu/globals/tutorials/.
Volocity is particularly useful for visualization and analysis of 3-dimensional and 4-dimensional datasets. The software can be downloaded onto your own computer and accessed using PVIC licenses for Volocity via our license server. The $1/hour charge for license use helps to partially offset the annual cost of the software maintenance agreement, which provides you with unlimited help over the telephone from the expert Volocity support team at 1-866-310-0932. To register to use our Volocity license server, please contact the core manager at firstname.lastname@example.org.